Research project C4/10/004 (Research action C4)
The BCCM/LMBP plasmid collection contains about 1.900 recombinant plasmids that can be used as valuable tools for applied and fundamental molecular biological, immunological and biomedical research.
The collection divides these plasmids in two major groups:
Vector types, such as, but not limited to,
• cloning vectors
• basic expression vectors
• gateway expression vectors
• dicistronic expression vectors
• adenoviral expression vectors
• retroviral expression vectors
• yeast two-hybrid expression vectors
• yeast three-hybrid expression vectors
• reporter gene vectors
Expression vectors and gene inserts, incl. mutants, such as, but not limited to,
• cytokines (TNF, IL-1β, IL-2, IL-4, IL-5, IL-6, IFN-β, LT, …)
• cytokine receptors (TNF-R1, TNF-R2, Fas)
• GTP-binding genes (Cdc42, RhoA, Rac1)
• ubiquitin related genes (Ubc9, UbB)
• promoter reporter gene constructs and mutants (IL-6, NF-κB, caspase-11, E-cadherin, …)
• apoptosis signaling related genes (RIP1, TRADD, FADD, caspases, CrmA, Bcl-2 family members, …)
• NF-κB signaling related genes (IRAK1, IRAK4, RIP1, RIP2,TRAF2, IKKα, IKKβ, NEMO, p65, Gal4-p65, ABINs, A20, TAX1BP1, …)
• toll-like receptor signaling related genes (TLR3, MyD88, TRIF, Mal, TRAM, …)
• bacterial type III secretion system related genes (exoS, exoT, sopE, yopE, yopP, …)
• IRES sequences and IRES-transactivating factors (PTB, UNR, PITSLRE kinase, hnRNPC1/C2, …)
• N-glycosylation related genes (Angls2, Ppgls2, Scgls2, Trgls2, GlcNAc-TI, cbhI, TS)
• monoamino-oxidase genes (MAOA, MAOB)
• toxin-related genes (CNF1)
• lipoprotein related genes (APOA1)
• hairpin RNA expression plasmids for genome-wide gene silencing in Arabidopsis thaliana
All publicly available plasmids are described in full molecular detail and are submitted to quality control tests (purity, viability and authenticity).
BCCM/LMBP also offers a selection of recommended or required bacterial host strains and DNA libraries, from fungal to human origin.
