Research project NP/DD/35 (Research action NP)
For controlling the application of the European directives and regulations, and of the corresponding Belgian legislation, a program of control of residues, concerning mainly substances illegally used as growth promoters and veterinary drugs, including unauthorized substances which could be used as veterinary drugs, must be put into place every year and executed by Member States of the EU after approval by the Commission.
Beside the controls performed at random and planned in the framework of the national plan (Directive 96/23/EC), there are also directed controls, based on indications of illegal use or in case of forced slaughter.
For antibiotics and other growth inhibiting substances, a well suited control system should consist of at least four stages:
1- Prescreening at the level of the slaughterhouses by means of a microbiological test (e.g. Belgian Kidney Test) for the presence of growth inhibiting substances. This phase is already fully operational.
2- Selective screening of the positives, obtained sub 1, by means of immunoassays in order to come to an identification of the group of growth inhibitors .
3- Chemical identification within the group of the individual growth inhibitors. Suitable techniques are: gas chromatography (GC), gas chromatography coupled to mass spectrometry (GC/MS), high performance liquid chromatography with ultra-violet detector (HPLC/UV) and HPLC coupled to mass spectrometry (HPLC/MS).
4- Quantitative assay of the identified residue in view of the established Maximum Residue Limit (MRL) (Council Regulation 2377/90).
The three later phases are not yet operational, due to the lack of an integrated analytical strategy.
This project aims at demonstrating the feasibility of such an integrated strategy.
The strategy will be based on the availability of appropriate immunoassays or other biochemical tests and of suitable chemical methodology, which is considered commonly available to a well equipped control laboratory.
A pilot methodology for the identification, the confirmation and quantitative determination of residues of commonly encountered antibacterial substances in animal tissues and products will be set up. After thorough validation in agreement with the internationally accepted standards (for example: Decision of the Commission 93/256/ECC), the methodology will be made available under the form of standard operating procedures (SOP).
This project represents the second part of a research project, granted by the OSTC, initiated on March 1, 1997, and entitled: Standardisation of hormone and veterinary drug residue analysis in animal products.
Stratégie intégrée d'analyse qualitative et quantitative des résidus de substances antimicrobiennes dans les denrées alimentaires: rapport final
Bruxelles: SSTC, 2001 (SP0829)
[To download]
Stratégie intégrée d'analyse qualitative et quantitative des résidus de substances antimicrobiennes dans les denrées alimentaires: annexes au rapport de l'ULg
Bruxelles: SSTC, 2001 (SP0830)
[To download]
Stratégie intégrée d'analyse qualitative et quantitative des résidus de substances antimicrobiennes dans les denrées alimentaires: annexes au rapport de la RUG
Bruxelles: SSTC, 2001 (SP0831)
[To download]
Geïntegreerde strategie voor de kwalitatieve en kwantitatieve residu-analyse van antibiotica in voedingsmiddelen van dierlijke oorsprong: gedetailleerd verslag RUG
Brussel: DWTC, 2001 (SP0832)
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Stratégie intégrée d'analyse qualitative et quantitative des résidus de substances antimicrobiennes dans les denrées alimentaires: résumé opérationnel
Bruxelles: SSTC, 2001 (SP0833)
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Stratégie intégrée d'analyse qualitative et quantitative des résidus de substances antimicrobiennes dans les denrées alimentaires: executive summary
Bruxelles: SSTC, 2001 (SP0834)
[To download]
